AUC 2022

Comprehensive multi-wavelength size distribution analysis of recombinant adeno associated virus vectors with Sedit

Presenter: Susumu Uchiyama, Department of Biotechnology, Graduate School of Engineering, Osaka University,
Manufacturing Technology Association of Biologics, Japan


Detailed, comprehensive and reliable characterization of virus vectors are required to ensure their efficacy and safety. We have been focusing on the development of methods for primary and higher order structure of recombinant adeno associated virus vectors (rAAVs). The size distribution analysis of rAAVs can be achieved with constituent components by multiwavelength sedimentation velocity analytical ultracentrifugation using OPTIMA AUC, by which we can determine the population of free DNA, intermediate particles, aggregates as well as full and empty particles.

Reference: Maruno T, Usami K, Ishii K, Torisu T, Uchiyama S. Comprehensive Size Distribution and Composition Analysis of Adeno-Associated Virus Vector by Multiwavelength Sedimentation Velocity Analytical Ultracentrifugation. J Pharm Sci. 2021 Oct;110(10):3375-3384. doi: 10.1016/j.xphs.2021.06.031. Epub 2021 Jun 27. PMID: 34186069.

This workshop will be presented on Sunday from 17:00-19:00 (session 4).