AUC 2022

Small Angle Scattering with UltraScan SOMO

Presenters: Saeed Mortezazadeh, University of Lethbridge, Canada (in-person), Emre Brookes, University of Montana, USA (remote), and Mattia Rocco, Retired, Ospedale Policlinico San Martino, Genova, Italy (remote)


The UltraScan Solution Modeler (US-SOMO)[1] has, in addition to hydrodynamic parameter calculation from structure, native and wrapped tools for computation of simulated biological solution small angle scattering (BioSAS) data from structure, processing of BioSAS data and comparison of simulated data against experimental data. US-SOMO also contains powerful tools for the analysis of size exclusion coupled small angle X-ray scattering (SEC-SAXS) data [2]. In this two session workshop, we will begin with an introduction to BioSAS. This will begin with an overview of the basics of BioSAS and its importance to the researcher. Next, during the first session, we will introduce the attendees to the available BioSAS tools within US-SOMO. In the second sessions, the attendees will work through hands-on exercices using US-SOMO with a focus on the SEC-SAXS tools.

It is suggested that attendees attend both sessions, as earlier material will not be covered again during the 2 session course. Registered attendees will receive detailed software installation instructions in the week prior to the workshop.

This workshop will be presented on Monday from 11:30-13:30 and 14:30-16:30 (sessions 6 and 7).

[1] Brookes, E. and Rocco, M., 2018. Recent advances in the UltraScan SOlution MOdeller (US-SOMO) hydrodynamic and small-angle scattering data analysis and simulation suite. European Biophysics Journal, 47(7), pp.855-864. doi: 10.1007/s00249-018-1296-0.

[2] Brookes, E., Vachette, P., Rocco, M. and PĂ©rez, J., 2016. US-SOMO HPLC-SAXS module: dealing with capillary fouling and extraction of pure component patterns from poorly resolved SEC-SAXS data. Journal of applied crystallography, 49(5), pp.1827-1841. doi: 10.1107/S1600576716011201