Challenges of nanoparticle analysis with AUC
Presenter: Lukas Dobler and
Rose Rosenberg, University of Konstanz, Germany
Description:Analytical Ultracentrifugation (AUC) was originally invented for the analysis of nanoparticles. It is still a great tool for their analysis. However, Nanoparticles pose special challenges to the researcher, which are typically not encountered in the analysis of proteins. These are:
- Polydispersity
- Hybrid character and therefore folded particle size and particle density distribution
- Stabilization by charge, which cannot be shielded by buffers
- A size which already leads to light scattering superimposing the signal by absorption
- Shape distributions
- Size dependent optical properties