Intro to SEDNTERP, a handy tool for AUC data interpretation
Presenters: John Philo Alliance Protein Laboratories
DescriptionProgram overview and hands-on software workshop Prerequisites: none (suitable for novice AUC users)
- Introduction to SEDNTERP and its use for calculating:
- solvent density, viscosity, and refractive index
- sample partial specific volume, hydration, and specific refractive increment (dn/dc)
- standardized sedimentation or diffusion coefficients (s20,w or D20,w) from raw experimental values
- sedimentation or diffusion coefficients extrapolated to zero concentration, and their concentration-dependence coefficients ks and kD
- hydrodynamic parameters from SV or diffusion results:
- anhydrous sphere radius, and corresponding maximum possible sedimentation and diffusion coefficients
- sample frictional coefficient, f/f0 ratio, and Stokes radius
- sample overall hydrodynamic shape and hydration characteristics (max hydration, Perrin P function, actual molecular size and shape for ellipsoid or cylinder models)