AUC 2022

Addressing Solution Complexity with the AUC: Polymers, Micelles, Nanocarriers

Presenters: Ivo Nischang, Laboratory of Organic and Macromolecular Chemistry (IOMC) and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Germany


  1. Basic relations
      (Intrinsic) sedimentation coefficients, (intrinsic) diffusion coefficients, partial specific volumes, translational friction properties, molar masses
  2. Sedimentation velocity experiments of polymers (synthetic and natural origin, hybrid systems)
    1. Sedimentation-diffusion analysis - Physically consistent values of the molar mass on par with alternative methods (size-exclusion chromatography / asymmetrical flow field-flow fractionation—multi-angle laser light scattering (SEC/AF4—MALLS); Hydrodynamic invariants
    2. (Self-)assemblies in solution - Degree of aggregation, stability against dilution, complexation/encapsulation of genetic material and drugs; Sizing when compared to dynamic light scattering (DLS)
    3. Nanoscale drug delivery systems - Resolving solution complexity including nanoparticles (NPs), encapsulated and free drug, targeting dye moieties, presence and absence of surfactants, colocalization studies
  3. Sizing aspects of nanocarriers and NPs (AUC, DLS, SEC/AF4—MALLS)
      Comparing hydrodynamic size estimates based on sedimentation analysis (AUC), sedimentation-diffusion analysis (AUC), diffusion measurements (DLS), and radii of gyration (MALLS)
  4. Identification of dynamic erosion processes of nanocarriers in solution
      Investigation of NPs in complex media containing protein and blood serum components, impact of temperature
  5. Current practical limits

    This workshop will be presented on Sunday from 11:30-13:30 (session 2), and on Monday from 14:30-16:30 (session 7).