| Submitter: |
Borries Demeler |
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| Authors: |
Amy Henrickson, Jay Kulkarni, Josh Zaifman, Gary Gorbet, Peter Cullis, Borries Demeler
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| Corresponding Author: |
Borries Demeler |
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| Title: |
Density Matching Multi-Wavelength Analytical Ultracentrifugation to Measure Drug Loading of Lipid Nanoparticle Formulations |
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| Contribution Type: |
Full Talk |
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| Selected for Presentation |
Yes |
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| Abstract: |
Like viral vectors, lipid nanoparticle formulations can be characterized by multi-wavelength analytical ultracentrifugation in high resolution to differentiate heterogeneous mixtures based on nucleic acid loading state, morphology, and particle size. In this talk, I will discuss how a D2O density matching approach can be employed to characterize particles based on their partial specific volume, or loading density. This adds a third characterization dimension to the hydrodynamic and spectral separation. |
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