| Submitter: |
Amy Henrickson |
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| Authors: |
Amy Henrickson, Gary E. Gorbet, Alexey Savelyev, Sarah K. Schultz, Xiaozhe Ding, Viviana Gradinaru, Borries Demeler
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| Corresponding Author: |
Amy Henrickson |
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| Title: |
Multi-wavelength Analytical Ultracentrifugation Methods to Identify Biopolymer Interactions |
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| Contribution Type: |
Full Talk |
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| Selected for Presentation |
Yes |
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| Abstract: |
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development, suitable for a wide range of applications and biopolymer systems. It adds an orthogonal spectral dimension to the traditional hydrodynamic characterization by exploiting unique chromophores in analyte mixtures, allowing the user to identify each spectrally different analyte and understand their potential interactions. Here we explain the improvement in resolution and information content obtained by this technique compared to traditional single- or dual-wavelength approaches for systems such as protein-nucleic acid interactions, protein-protein studies, and mixtures of loaded and empty adeno-associated viruses. |
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