AUC 2022

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SEDNTERP version 3: An improved tool for both AUC and light scattering
Submitter: John Philo
Authors: John Philo
Corresponding Author: John Philo
Title: SEDNTERP version 3: An improved tool for both AUC and light scattering
Contribution Type: Full Talk
Selected for Presentation Yes
Abstract: This talk will highlight some new features of SEDNTERP version 3, including:
1. Buffer properties calculated from the buffer composition now include the refractive index.
2. Buffer component concentrations can now be entered in units of mg/mL, g/mL, molal, or weight %.
3. Sample properties calculated from the sample composition now include the specific refractive increment dn/dc (which is adjusted based on the buffer refractive index and the measurement wavelength).
4. The conjugates table now includes entries for a generic glycan (to create glycoproteins) as well as a 10 kDa PEG (for creating PEGylated proteins with any PEG mass). Those entries include refractivity data, so dn/dc values for glyco- or PEGylated proteins can also be calculated.
5. SEDNTERP now handles diffusion experiments in addition to sedimentation velocity and equilibrium. These 3 types of experiments now have separate types of records in the user database.
6. Sample hydrodynamic properties can be calculated from either sedimentation or diffusion coefficients (when both are available), and results from separate sedimentation and diffusion experiments can be combined to derive the sample molar mass (even when those experiments were done in different buffers or at different temperatures).
7. A new tool is available for extrapolating sedimentation or diffusion coefficients measured at multiple concentrations to zero concentration, while also determining the ks or kD coefficient. The concentration series data can also be stored in a new type of record in the user database.
8. The derived sample hydrodynamic properties now include the Perrin P function and the fhyd/f0 ratio, to provide clearer separation between effects of molecular shape vs. hydration.